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HTRF mAb Anti-Phospho-CREB (Ser133) Eu-Conjugate, 500 Assay Points

MAb Anti-phospho CREB-Eu cryptate enables the rapid detection of CREB phosphorylation on Ser133 in a biochemical kinase assay format.

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Feature	Specification
Application	Biochemical Enzymatic Assay

MAb Anti-phospho CREB-Eu cryptate enables the rapid detection of CREB phosphorylation on Ser133 in a biochemical kinase assay format.

View product information

Product Variants

Unit Size: 100,000 Assay Points

Part #:

61P13KAY

Unit Size: 500 Assay Points

Part #:

61P13KAE

Unit Size: 10,000 Assay Points

Part #:

61P13KAZ

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

The Anti-phospho CREB-Eu cryptate is a labeled monoclonal Ab enabling the rapid detection of CREB only when phosphorylated at Ser133. It does not cross-react with the corresponding non-phosphorylated sequence and also detects the phosphorylated form of the CREB-related protein ATF-1. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Other Specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Molecular Modification	Phosphorylation
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	Kinase
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF MAb Anti-phospho CREB-Eu cryptate 1.svg

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho CREB-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.