メニュー
Request a Quote
お問い合わせ
JP

HTRF KRAS G12C / SOS1 Binding Kit Standard

HTRF KRAS/SOS1 binding standard image
Video Play Button
HTRF KRAS G12C / SOS1 Binding Kit Standard
HTRF KRAS/SOS1 binding standard image
Video Play Button
HTRF KRAS/SOS1 binding standard image
HTRF KRAS/SOS1 binding standard
HTRF KRAS/SOS1 binding standard image
HTRF KRAS/SOS1 binding standard image
Video Play Button

The KRAS/SOS1 binding Standard is intended for use with the KRAS WT/SOS1 and KRAS G12C/SOS1 binding kits.

View product information
Feature Specification
Application Protein-Protein Interaction

The KRAS/SOS1 binding Standard is intended for use with the KRAS WT/SOS1 and KRAS G12C/SOS1 binding kits.

View product information
Product Variant
Unit Size: 1 vial
Part #:
64KRASPPICDA
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Request More Information
HTRF KRAS/SOS1 binding standard image
HTRF KRAS/SOS1 binding standard image
Video Play Button
HTRF KRAS G12C / SOS1 Binding Kit Standard
HTRF KRAS/SOS1 binding standard image
Video Play Button
HTRF KRAS/SOS1 binding standard image
HTRF KRAS/SOS1 binding standard
HTRF KRAS G12C / SOS1 Binding Kit Standard
HTRF KRAS/SOS1 binding standard image
HTRF KRAS/SOS1 binding standard image
Video Play Button

Product information

Overview

KRAS/SOS1 binding standard can be used as a standard to control assay reproducibility. KRAS/SOS1 binding d is intended for use with the KRAS WT/SOS1 and the KRAS G12C/SOS1 kits.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Standard
Shipping Conditions
Shipped in Dry Ice
Target Class
Binding Assay
Technology
TR-FRET
Therapeutic Area
Oncology & Inflammation
Unit Size
1 vial

Video gallery

Video Play Button
HTRF KRAS G12C / SOS1 Binding Kit Standard
Video Play Button
HTRF KRAS G12C / SOS1 Binding Kit Standard
Video Play Button

How it works

Assay principle

The KRAS/SOS1 PPI assays include tagged human recombinant partners (KRAS WT or G12C and SOS1) and labelled anti-tag reagents for HTRF detection. Without any inhibitor, KRAS loaded with GTP binds to SOS1 , and the binding of each detection reagent to its tagged target generates an HTRF signal. In the presence of KRAS/SOS1 inhibitors or GTP competitors, the HTRF signal decreases.

ppi-how-it-works-assay-principle-kras-g12c-sos1

 

Assay protocol

The Human KRAS/SOS1 binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, compounds or standards are dispensed directly into the assay plate. The pre-mixed GTP and human Tag1-KRAS protein is then added, together with the human Tag2-SOS1 protein, followed by the dispensing of the HTRF reagents, the anti-Tag2 antibody labeled with Terbium cryptate and the anti-Tag1 antibody labeled with XL665. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

ppi-how-it-works-assay-protocol-kras-wt-sos1

 

Assay validation

Standard curves with human KRAS WT and G12C

The KRAS/SOS1 binding standard inhibits the KRAS/SOS1 interaction for KRAS WT and KRAS G12C proteins in a correlated manner.

assay-validation-human-htrf-kras-sos1-std

 

Resources

Are you looking for resources, click on the resource type to explore further.

1-1 of 1 Resources
Technical Note Icon
Technical Note
How to access true Kd values in Protein-Protein Interaction assays

Investigate the dynamics of Interactions

Kinetic values are of importance for characterizing protein-protein interactions...

Learn More

Loading...

Scroll Icon