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HTRF Human PCSK9 Detection Kit, 10,000 Assay Points

The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

The Human PCSK-9 kit is designed for the rapid quantification of Proprotein convertase subtilisin/kexin type 9 in cell supernatants.

View product information

Product Variants

Unit Size: 500 Assay Points

Part #:

63ADK050PEG

Unit Size: 10,000 Assay Points

Part #:

63ADK050PEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

PCSK-9 (proprotein convertase subtilisin/kexin type 9) is a member of the proprotein convertase family that plays an important role in lipoprotein homeostasis. PCSK9 binds to the receptor for low-density lipoprotein particles (LDLR) within extracellular fluid, triggering ingestion of LDL-particles into cells. PCSK9 blocking can lower blood LDL-particle concentrations. The PCSK-9 kit is designed for the detection of proprotein convertase subtilisin/kexin type 9 directly in cell supernatants.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Cardiovascular Metabolism/Diabetes
Unit Size	10,000 Assay Points

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How it works

Assay principle

Human PCSK-9 is measured using a sandwich immunoassay involving two specific human PCSK-9 antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of PCSK-9 present in the sample.

Assay Protocol

The PCSK-9 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 2-hour incubation period at RT.