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HTRF Human Pro-Collagen Type 1 Detection Kit, 500 Assay Points

The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.

View product information

Product Variants

Unit Size: 500 Assay Points

Part #:

63ADK014PEG

Unit Size: 10,000 Assay Points

Part #:

63ADK014PEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

Human pro-collagen type 1 is made by a combined association of two pro-alpha1 chains with a pro-alpha2 chain. These triple-stranded, rope-like pro-collagen molecules must be processed by enzymes outside the cell to form fibrils that cross-link to produce strong mature type I collagen fibers. The human pro-collagen type 1 kit is designed for the quantitative detection of human pro-collagen type 1, using cell supernatants, whole cells, or recombinant protein extracts.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biomarkers
Target Species	Human
Technology	TR-FRET
Therapeutic Area	NASH/Fibrosis Rare Diseases
Unit Size	500 Assay Points

Video gallery

How it works

Assay principle

Human pro-collagen is measured using a sandwich immunoassay involving two specific anti-human pro-collagen antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of pro-collagen present in the sample.

Assay protocol

The human pro-collagen type 1 assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection of human pro-collagen by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.