HTRF HIV p24 Detection Kit, 500 Assay Points

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HTRF HIV p24 Detection Kit, 500 Assay Points

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The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Dynamic Range	33 - 10,000 pg/mL
Limit of Detection	12 pg/mL
Sample Volume	16 µL

The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.

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Product Variants

Unit Size: 500 Assay Points

Part #:

64P24PEG

Unit Size: 10,000 Assay Points

Part #:

64P24PEH

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Product information

Overview

Immunodeficiency Virus (HIV) p24 is the 231 amino acid phosphorylated protein of the capsid forming the conical core of the virus, which encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the p55 Gag polyprotein by viral proteases. HIV p24 and its 55 kDa precursor play crucial roles in the assembly, maturation, and disassembly of HIV. e therapy, HIV-derived lentiviral vectors are useful and commonly implemented to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Dynamic Range	33 - 10,000 pg/mL
Limit of Detection	12 pg/mL
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biologics
Technology	TR-FRET
Therapeutic Area	Infectious Diseases
Unit Size	500 Assay Points

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Assay details

Key features

16 µL of recombinant protein in diluent #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti HIVp24 detection reagents. The HTRF signal was recorded after 2h incubation at RT.

Sample size	16 µL
Final assay volume	20 µL
Time to results	2h at RT
Detection limit	12 pg/mL
Dynamic range	33 to 10,000 pg/mL
Sample compatibility	Cell supernatant