Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong (China) *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

HTRF Estradiol Detection Kit, 500 Assay Points

The Estradiol kit is intended to quantify 17b-estradiol from cells, serum, and in biochemical assays.

View product information

Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

The Estradiol kit is intended to quantify 17b-estradiol from cells, serum, and in biochemical assays.

View product information

Product Variants

Unit Size: 500 Assay Points

Part #:

62ESTPEG

Unit Size: 10,000 Assay Points

Part #:

62ESTPEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Request More Information

Product information

Overview

There is substantial research indicating that breast cancer tissue contains the enzymes necessary to synthesize estradiol (17ß-estradiol), a steroid hormone that plays an important role in this disease. Two main pathways are involved in the formation of estradiol in breast cancer tissues: the aromatase pathway, which transforms androgens into estrogens, and the sulfatase pathway, which converts estrone into estradiol via the 17ß-HSD1 enzyme. The Estradiol kit is a highly specific assay for measuring estradiol in cell supernatants, or biochemical enzyme assays.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	Biomarkers
Technology	TR-FRET
Therapeutic Area	Cardiovascular Metabolism/Diabetes
Unit Size	500 Assay Points

Video gallery

Citations

View product citations for 62ESTPEG on CiteAb

How it works

Assay principle

The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and estradiol coupled to XL665 (acceptor). Estradiol produced by cells or purified 17ß-HSD1 enzyme competes with XL665-labelled estradiol for binding to monoclonal anti-estradiol Eu Cryptate.

Assay protocol

The estradiol assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Estradiol detection using HTRF reagents This protocol requires only a single, two-hour incubation period following cell stimulation.

Assay details

Key features

Detection limit:	0.02 ng/mL
Dynamic range:	0.02 to 20 ng/mL
EC50:	0.8 ng/mL
S/B:	14
Specificity:	Estrone 0.4% Estriol 0.3% Estrone 3 sulfate 0.002% Testosterone 0.2% Progesterone 0.002%.

Assay validation

Detecting 17ß-HSD1 activity

A human breast cancer cell line, T-47D, was tested for the production of estradiol when stimulated with estrone using the HTRF Estradiol assay. Assay reagents were added directly to the cell culture plate following cell stimulation. As Figure 2 shows, estradiol was detected using as few as 780 cells. The ability to miniaturize and run the assay in a single plate while not sacrificing assay performance makes HTRF Estradiol an ideal tool for screening.

T-47D cells were dispensed as indicated into 96-half-well plates. 50 µl MEM containing 10 nM Estrone were added and cells incubated for 20 hours. Estradiol assay reagents were added directly to cultured cells. Following a 2 hour incubation, fluorescence signals were read and estradiol concentrations calculated using a standard curve.