Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong (China) *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points

The CD32a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32a receptor on live cells.

View product information

Feature	Specification
Application	Protein Quantification
Sample Volume	5 µL

The CD32a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD32a receptor on live cells.

View product information

Product Variants

Unit Size: 100 Assay Points

Part #:

6FRAHPAG

Unit Size: 500 Assay Points

Part #:

6FRAHPAE

Unit Size: 1,000 Assay Points

Part #:

6FRAHPAF

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Request More Information

Product information

Overview

The CD32a (FcgRIIa, H131) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32a receptorin live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Phagocytosis (ADCP). Two separate CD32a assays are available to specifically target the H131 or R131 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	5 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biologics
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Rare Diseases
Unit Size	100 Assay Points

Video gallery

How it works

Assay principle

The CD32a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32a variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabeled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.

Assay protocol

The CD32a receptor cellular binding assays (FcgIIa H131 or R131) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.