HostDetect E.coli PCR DNA Quant Kit

The HostDetect™ E.coli PCR DNA Quant Kit is specific for DNA from E.coli genome. The reagents utilize sequence-specific primers and TaqMan® probe to amplify the 16S ribosomal RNA gene of E.coli genomic DNA for residual host genomic identification. A primer/probe set to detect Internal Control (IC), is also included for monitoring entire process from extraction to real-time PCR. The reagents also use a dUTP/UNG carryover prevention system to avoid contamination of PCR products. For more information, contact: BioprocessQC@revvity.com

Key Highlights:

• Quantify E.coli genomic DNA with as little as 0.03 pg per PCR reaction in < 2 hours of PCR
• Internal control detection for process monitoring from sample extraction to PCR
• High specificity: No cross-reactivity with unrelated DNA
• Flexible extracted DNA input volume up to 10 µL per well
• Scalable reaction numbers from 1 to up to 192 per kit