HostDetect CHO PCR DNA Quant Kit

The HostDetect™ CHO PCR DNA Quant Kit is specific for DNA from CHO genome and not subject to detection of human or environment DNA that might be introduced during sample handling.

The reagents utilize sequence-specific primers and TaqMan® probe to amplify the Alu-equivalent gene of CHO genomic DNA for residual host genomic identification. A primer/probe set to detect Internal Control (IC), is also included for monitoring entire process from extraction to real-time PCR. The reagents also use a dUTP/UNG carryover prevention system to avoid contamination of PCR products. For more information, contact: BioprocessQC@revvity.com

Key Highlights:

• Quantify CHO genomic DNA with as little as 0.01 pg per PCR reaction in < 2 hours of PCR
• Internal control detection for process monitoring from sample extraction to PCR
• High specificity: No cross-reactivity with unrelated DNA
• Flexible extracted DNA input volume up to 10 µL per well
• Scalable reaction numbers from 1 to up to 192 per kit