GrowDex-T is a transparent negatively charged hydrogel that consists of nanofibrillar cellulose (1.0% w/v) and 99.0% purified water and is supplied ready to use. The transparent nature of GrowDex-T offers superior imaging properties, makes it ideal for use in brightfield, phase contrast and confocal microscopy applications as well as high content imaging. GrowDex-T is ready to use and can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient temperature handling and shear thinning properties of GrowDex-T enable easy handling and use in automated dispensing systems. The product is supplied sterilized.

Benefits:

• Animal-free: No animal biomolecules interfering with readouts.
• Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
• Reliable: robust and sustained performance regardless of the production lot ordered.
• Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
• Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
• Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
• Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

• Storage: Ambient
• Shelf life: 12 months
• Shelf life after opening: 3 months

Concentration

• GrowDex-T is supplied at a concentration of 1.0%.
• Working concentrations are usually between 0.2% and 0.8%.