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GrowDex 2.5 ml

GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

For research use only. Not for use in diagnostic procedures.

GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

For research use only. Not for use in diagnostic procedures.

Overview

GrowDex has been designed to provide a support matrix for the culture of cells in a wide range of applications. It comprises of two components, nanofibrillar cellulose (NFC) and ultra-pure water. The length of the nanofibrils ranges from 200-300 μm, with a diameter in the nanometer scale varying from 5-100 nm.

The product is supplied sterilized, ready to use and can be utilized in a variety of cell culture applications, such as 3D spheroid or organoid formation.

Benefits:

  • Animal-free: No animal biomolecules interfering with readouts.
  • Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
  • Reliable: robust and sustained performance regardless of the production lot ordered.
  • Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
  • Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
  • Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
  • Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

  • Storage: Ambient
  • Shelf life: 12 months
  • Shelf life after opening: 3 months

Concentration

  • GrowDex is supplied at a concentration of 1.5%.
  • Working concentrations are usually between 0.2% and 1.0%.

GrowDase™ enzyme breaks down GrowDex hydrogel to form a solution in an easy one step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.

Specifications

Concentration
GrowDex is supplied at a concentration of 1.5%<br>Working concentrations are usually between 0.2% and 1.0%
Form
Hydrogel
Brand
GrowDex®
Quantity
1 x 2.5 mL
Shipping Conditions
Shipped Ambient
Storage Conditions
Ambient

Resources

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1-6 of 22 Resources
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Brochure
3D cell culture workflow solutions

More than ever, researchers are turning to 3D cell cultures, microtissues and organoids to bridge the gap between 2D cell cultures...

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Application Note
3D in vitro culture of the neuronal cell line SH-SY5Y is sustained in GrowDex®

Here we establish methodologies for 3D cell culture of the common neuronal cell line SH-SY5Y within the GrowDex® hydrogel to...

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Application Note
Adipose derived mesenchymal stem cells: Investigating sphere formation in relation to seeding density and hydrogel concentration

Here, adipose derived MSCs (ADMSCs) were cultured in nanofibrillar cellulose, GrowDex®, under various conditions for 1 week to...

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Application Note
Breast Cancer Cell Line MCF7 3D Culture Demonstrated in GrowDex® and Matrigel

In this study, the morphology development of MCF7 cells in wood-derived nanofibrillar cellulose hydrogel GrowDex® (3D), and in...

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Application Note
Evaluating HTRF and AlphaLISA SureFire Ultra detection methods in GrowDex-supported 3D MCF7 cell cultures

In our application note, we evaluate the compatibility of GrowDex, a wood-derived nanofibrillar cellulose hydrogel 3D cell culture...

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Product Info
GrowDase Instructions

This data sheet provides instructions for use for GrowDase cellulase enzyme.

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