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DELFIA Europium, 200mL (Cytotoxicity Detection Only)

DELFIA^® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.

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DELFIA^® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.

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Product Variant

Unit Size: 200 mL

Part #:

C135-100

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

The DELFIA europium solution is intended for use in cell-mediated DELFIA cytotoxicity assay. It forms part of the AD0116 DELFIA^® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA Eu-solution is sufficient for 10x96 wells.

DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a
hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.