Why Use DOPlify Technology?

The DOPlify WGA V2 kit is a whole genome amplification kit designed to PCR amplify the genome from single cells and limited DNA templates.

The DOPlify kit is unique in that it uses advanced DOP-PCR, which;

• uses the high processivity and fidelity of new generation polymerases that are NGS application grade
• uses ready-mixes to minimize the pipetting steps and requiring only two sample tube openings to reduce the risk of contamination
• provides an amplification system that is flexible enough to concurrently whole genome amplify a sample while specifically amplifying genes of interest, reducing the risk of allele drop-out.

DOPlify workflow

Can DOPlify WGA V2 be automated?

The whole genome amplification of single or small numbers of cells and downstream processes such as library preparation for NGS can be fully automated with the JANUS G3 liquid handler.

Whole genome amplification (WGA) of single cells in tubes has traditionally been performed by hand, which can lead to multiple potential issues including:

• Errant volume pipetting
• Technologist fatigue
• Wasted time
• Sample contamination

Using the JANUS G3 liquid handler with preprogrammed, easy-to-use automated WGA protocols your lab can:

• Reduce human errors and contamination risk
• Gain time for your staff to perform more important tasks
• Increase your lab’s throughput with walkaway automation
• Ensure sample process traceability with barcode scanning and sample tracking

Revvity’s JANUS G3 liquid handler can be used to automated whole genome amplification using the DOPlify kit or other WGA technologies with a throughput of 48 - 96* samples per run.

* Higher throughput options are available

• Deleye, L., Tilleman, L., Plaetsen, A. V., Cornelis, S., Deforce, D., & Nieuwerburgh, F. V. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-03711-y.
• Deleye, L., Plaetsen, A. V., Weymaere, J., Deforce, D., & Nieuwerburgh, F. V. (2018). Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Scientific Reports,8(1). doi:10.1038/s41598-018-19509-5.
• Mai, A. D., Harton, G. L., Quang, V. N., Van, H. N., Thi, N. H., Thuy, N. P., . . . Quoc, Q. T. (2020). Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. doi:10.1007/s10815-020-02006-y.
• Plaetsen, A. V., Deleye, L., Cornelis, S., Tilleman, L., Nieuwerburgh, F. V., & Deforce, D. (2017). STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods. Scientific Reports, 7(1). doi:10.1038/s41598-017-17525-5.