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CRISPRClean Plus Bulk Ribodepletion Reagents (Human, Mouse, Rat, Pan Bacteria)

Revolutionize your RNA-seq analysis by purging excessive rRNA from human, mouse, rat, and bacterial samples. Our cutting-edge protocol is crafted for high-throughput applications by enabling depletion on multiplexed pools of samples.

Feature Specification
Automation Compatible No
Product Group Ribodepletion

Revolutionize your RNA-seq analysis by purging excessive rRNA from human, mouse, rat, and bacterial samples. Our cutting-edge protocol is crafted for high-throughput applications by enabling depletion on multiplexed pools of samples.

Product Variant
For research use only. Not for use in diagnostic procedures.
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Overview

CRISPRClean: Precision rRNA Depletion for Complex Samples

Harness the specificity of CRISPRclean reagents to discern and reduce ribosomal RNA from mixed bacterial and human cell samples. This innovative approach not only streamlines rRNA removal but also purges non-essential bacterial sequences pre-sequencing, reallocating over 80% of reads towards the detection of lower expressing, biologically relevant transcripts.

Key highlights:

  • Optimal for high-throughput transcriptome profiling
  • Efficiently process up to 16 RNA-seq libraries simultaneously
  • Remove up to 80% of both eukaryotic and bacterial rRNA reads.
  • Simplified protocol compatible with automated systems.

The CRISPRClean™ Bulk Ribodepletion Reagents (Human, Mouse, Rat, Pan Bacteria) is used after the completion of standard RNA-seq library preparation protocols. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX® Rapid Directional RNA-seq kit 2.0.

Specifications

Automation Compatible
No
Product Group
Ribodepletion
Shipping Conditions
Shipped in Dry Ice
Unit Size
256 samples, 16 reactions

References

  1. Uranga C, Nelson KE, Edlund A and Baker JL (2022) Tetramic Acids Mutanocyclin and Reutericyclin A, Produced by Streptococcus mutans Strain B04Sm5 Modulate the Ecology of an in vitro Oral Biofilm. Front. Oral. Health 2:796140. doi: 10.3389/froh.2021.796140.

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