chemagic miRNA 200 Kit H96

The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.

With only 10 minutes of hands-on time within a 90-minute total processing time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.

“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”

Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki