Cellometer K2 Fluorescent Cell Counter

Predefined assays and cell types

Take the guesswork out of setting up your cell quantification experiments. The Cellometer K2 includes a library of commonly-used assays and cell types with predefined settings to help improve consistency of results. Conveniently build and modify assays and cell types to help match your specific experimental requirements.

Capture multiple fields of view

Capture one, four, or eight images per sample. The instrument default is set to four images, which is the equivalent of six quadrants on a hemocytometer. Eight images are equivalent to twelve quadrants on a hemocytometer. The ability to capture multiple images improves the cell counting dynamic range and reliability of results.

Dual-fluorescent staining for clumpy cells

The fluorescent image shows bright green AO-positive hepatocytes declustered by the Cellometer K2 algorithm. Red circled hepatocytes are PI-positive (dead) while free nuclei are not counted.

An optional module can be purchased to facilitate 21 CFR Part 11 compliance. The additional module comes with the following features:

• User login with passwords
• User assigned permissions
• Audit trail
• Error log files
• Electronic signatures

Why dual-fluorescence?

Because brightfield cell counting does not differentiate nucleated from non-nucleated cells and Trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for reliable viability analysis for primary cells. The K2 is equipped with standard assays for dual-fluorescence analysis of a variety of cells stained with Acridine Orange and Propidium Iodide (AO/PI).

The AO/PI method

Acridine Orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium Iodide (PI) can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

Imaging / counting chambers: no washing or contamination

We offer two different disposable counting slides, one with protective coverings on both sides and a ready-to-use option packed in microscope slide boxes. Disposable slides offer the advantages of time-saving, since no washing is required, reduced risk of contamination and reduced biohazard risk to users.