AlphaScreen FLAG Detection Kit, 500 Assay Points

This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume. The M2 antibody clone can recognize N-terminal, C-terminal, and internal FLAG tags.

This bead kit can be used for many applications, including:

• Protein-protein interaction assays
• Protein-peptide interaction assays
• FLAG-tagged protein detection and quantitation
• Protein expression
• Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is FLAG-tagged, and associates with the AlphaScreen anti-FLAG Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FLAG probe provided in the kit.