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  • AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points

AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points

AlphaLISA Binding image
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AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points
AlphaLISA Binding image
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AlphaLISA Binding image
AlphaLISA Binding
AlphaLISA Binding image
AlphaLISA Binding image
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The AlphaLISA™ human TNFα/TNFR2 binding kit is designed for the detection of binding activity between human TNFα and TNFR2, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 5 µL

The AlphaLISA™ human TNFα/TNFR2 binding kit is designed for the detection of binding activity between human TNFα and TNFR2, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.

View product information
Product variants
Unit Size: 500 assay points
Part #:
AL3121C
Unit Size: 5,000 assay points
Part #:
AL3121F
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
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AlphaLISA Binding image
AlphaLISA Binding image
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AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points
AlphaLISA Binding image
Video Play Button
AlphaLISA Binding image
AlphaLISA Binding
AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points
AlphaLISA Binding image
AlphaLISA Binding image
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Product information

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  • Specifications
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Overview

The AlphaLISA™ human TNFα/TNFR2 binding assay uses anti-6xHis AlphaLISA Acceptor beads to capture the His-tagged TNFR2 and Streptavidin-coated donor beads to capture the biotinylated TNFα. Donor beads and Acceptor beads come into proximity through TNFα binding to TNFR2. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 20 µL reaction volume.
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 20 µL reaction volume.

AlphaLISA features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

Tumor necrosis factor alpha (TNFα) is an immunity-modulating cytokine that is required for defense against infectious diseases and carcinogenesis. TNFα activates signals through its two receptors [TNF receptor 1 (TNFRI) and TNF receptor 2 (TNFRII)]. TNFRI is ubiquitously expressed and can be fully activated by both the membrane-bound and soluble trimeric forms of TNFα, whereas TNFRII is found mostly on certain populations of immune cells and respond to the membrane-bound form of the TNFα homotrimer. However, a block of TNFα-mediated host defense often increases the risk of bacterial or viral infection or of the development of lymphoma. Thus, a thorough understanding of the function of the TNFα-TNFR binding is important for the design of optimal therapies against the various TNFα-related autoimmune diseases.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
TNFα,TNRFR3
Target Class
Binding Assay
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
5,000 assay points

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AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points
AlphaLISA Human TNF-α / TNFR2 Binding Kit, 5,000 Assay Points

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Application Note
Screening for inhibitors of PD-1 and PD-L1 binding with AlphaLISA technology

Many tumor cells, which under normal, healthy conditions would be recognized by the body’s T cells and thereby targeted for...

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