AlphaLISA Human NT-proBNP Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76-amino acid sequence cleaved from the N-terminal end of brain natriuretic peptide. NT-proBNP is an inactive protein that is used for the screening for acute congestive heart failure. Serum and plasma levels of NT-proBNP varies between individuals and generally increase with age. The physiological role of NT-proBNP is not clear, while the function of BNP is to protect the cardiovascular system from volume overload by regulating salt and water homeostasis. NT-proBNP has a longer biological half-life and therefore is the preferred biomarker for detection and analysis.