Skip to main content

AlphaLISA Human IFN-γ Detection Kit, 500 Assay Points

AlphaLISA™ Human IFN-γ Detection Kit is designed quantitative determination of human IFN-γ in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

We now offer a new and improved High Performance version of AlphaLISA Human IFN-γ Detection Kit, part number AL3153.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein Quantification
Dynamic Range 10.6 - 100,000 pg/mL
Limit of Detection 10.6 pg/mL  
Sample Volume 5 µL

AlphaLISA™ Human IFN-γ Detection Kit is designed quantitative determination of human IFN-γ in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

We now offer a new and improved High Performance version of AlphaLISA Human IFN-γ Detection Kit, part number AL3153.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
10.6 - 100,000 pg/mL
Host Species
Human
Limit of Detection
10.6 pg/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
IFNγ
Target Class
Cytokines
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

Video gallery

Citations

Resources

Are you looking for resources, click on the resource type to explore further.

1-1 of 1 Resources
Brochure Icon
Brochure
Detecting cytokines with AlphaLISA guide

Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run...

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Safety data sheet
+ Show more
Certificate of analysis
+ Show more
IFUs, Manuals & more
Scroll Icon