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AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total SIRPα assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total SIRPα assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Total SIRPα assay. This control is already provided in the AlphaLISA SureFire Ultra Human Total SIRPα assay kit but can also be ordered separately.
AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS, adjusted to 106 cells/mL and treated with 500 μM Pervanadate for 15 minutes. Cells were lysed with the addition of 5X Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SIRPα
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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