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AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total LCK assay. This control is already provided in the AlphaLISA SureFire Ultra Total LCK assay kit, but can also be ordered separately.

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Feature	Specification
Application	Protein Quantification

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Product Variant

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Total LCK Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from Jurkat cells, cultured in a T175 flask to approximately 1 x 106 cells/mL in 10% FBS containing medium. Cells were resuspended in HBSS at 4 x 106 cells/mL and treated with 11 mM Peroxide plus 50 nM Calyculin A for 10 minutes. Cells were lysed with 5X SureFire Ultra Lysis Buffer at a final density of 3.2 x 106 cells/mL.

Specifications

Other Specifications

Application	Protein Quantification
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Modality	Alpha
Lysis Buffer Compatibility	Lysis Buffer
Molecular Modification	Total
Product Group	Control Lysate
Shipping Conditions	Shipped in Blue Ice
Target	LCK
Target Class	Phosphoproteins
Target Species	Human
Technology	Alpha
Unit Size	1 Vial