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AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points

AlphaLISA Surefire Ultra Total Protein
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AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points
AlphaLISA Surefire Ultra Total Protein
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AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
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The AlphaLISA™ SureFire® Ultra™ Total IRF3 assay is a sandwich immunoassay for quantitative detection of total IRF3 in cellular lysates using Alpha Technology.

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Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Total IRF3 assay is a sandwich immunoassay for quantitative detection of total IRF3 in cellular lysates using Alpha Technology.

View product information
Product Variants
Unit Size: 500 assay points
Part #:
ALSU-TIRF3-A500
Unit Size: 10,000 assay points
Part #:
ALSU-TIRF3-A10K
Unit Size: 50,000 assay points
Part #:
ALSU-TIRF3-A50K
Unit Size: 100 assay points
Part #:
ALSU-TIRF3-A-HV
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
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AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
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AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points
AlphaLISA Surefire Ultra Total Protein
Video Play Button
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AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points
AlphaLISA Surefire Ultra Total Protein
AlphaLISA Surefire Ultra Total Protein
Video Play Button
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Product information

Overview

Interferon regulatory factor 3 (IRF3) is a part of the IRF family which is involved in the regulation of interferon α and β and play a role in host anti-viral immune regulation, cell growth, and hematopoietic development. In viral infected cells, phosphorylation of transcription factor IRF3 induces the production of antiviral and proinflammatory cytokines, a key element in the type 1 interferon-independent innate immune response. IRF3 is activated downstream of the STING signaling pathway and toll-like receptor signaling pathway. IRF3 is associated with cancer and autoinflammatory disease.

The AlphaLISA SureFire Ultra Total IRF3 assay is a sandwich immunoassay for the quantitative detection of total IRF3 in cellular lysates, using Alpha Technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Total
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
IRF3
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Autoimmunity
Oncology
Unit Size
50,000 assay points

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AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points
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AlphaLISA SureFire Ultra Human Total IRF3 Detection Kit, 50,000 Assay Points
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Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Guide
AlphaLISA SureFire Ultra: the ultimate guide for successful experiments

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay

Several biological processes are regulated by...

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