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AlphaLISA SureFire Ultra Human Phospho-DAP12 (Tyr91), Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-DAP12 (Tyr91) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-DAP12 (Tyr91) assay kit, but can also be ordered separately.

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Feature	Specification
Application	Cell Signaling

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Product Variant

Unit Size: 1 Vial

Part #:

ALSU-PDAP12-B-L

For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-DAP12 (Tyr91) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were seeded at 400K/mL and grown overnight in T175 flasks in medium containing 10% FBS. Cells were washed in HBSS and treated with 0.25 mM Pervanadate for 1 hour. Cells were lysed in SureFire Ultra Lysis Buffer at a final cell concentration of 5 x 10⁶cells/mL.

Specifications

Other Specifications

Application	Cell Signaling
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Modality	Alpha
Lysis Buffer Compatibility	Lysis Buffer
Molecular Modification	Phosphorylation
Product Group	Control Lysate
Shipping Conditions	Shipped in Blue Ice
Target	DAP12
Target Class	Phosphoproteins
Target Species	Human
Technology	Alpha
Therapeutic Area	Neuroscience
Unit Size	1 Vial