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AlphaLISA SureFire Biotin-Free Human Total SYK Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total SYK assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total SYK assay kit but can also be ordered separately.

Feature Specification
Application Cell Signaling
Protocol Time 2h at RT

Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total SYK assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total SYK assay kit but can also be ordered separately.

Product Variant
Unit Size: 1 vial
Part #:
ASBF-TSYK-A-L
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

This control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human Total SYK Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS + 0.1% BSA and adjusted to 2 x 106 cells/mL, then treated with 0.5 mM Pervanadate for 30 minutes. Cells were spun down at 300 RCF for 5 minutes and lysed in Lysis Buffer at 1 x 106 cells/mL.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Biotin-Free
Detection Modality
Alpha
Protocol Time
2h at RT
Shipping Conditions
Shipped in Blue Ice
Target
SYK
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Autoimmunity
Oncology
Unit Size
1 vial

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