Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total AKT1/2/3 assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total AKT1/2/3 assay kit but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Total AKT1/2/3 assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human Total AKT1/2/3 assay kit but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Biotin Free Human Total AKT1/2/3 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEK293 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 20% FBS for 15 minutes and lysed with 4 mL of Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Biotin-Free
|
Detection Modality |
Alpha
|
Protocol Time |
2h at RT
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
AKT1/2/3
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Metabolic
Oncology
|
Unit Size |
1 vial
|
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