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Membrane Target Systems: Adenosine A2B (human) Membrane Preparation

Cell membranes prepared from cells stably-transfected with Adenosine A2B (human) receptor, in HEK-293 cells.

Feature Specification
µg protein/unit 20 µg protein / unit
Concentration 8 µg/µL protein
G-Alpha Natural Receptor Gs
Host Cell Line HEK293
Host Species Human
Receptor Adenosine
Target Class GPCR
Validation Binding
Volume 1000.0 µL

Cell membranes prepared from cells stably-transfected with Adenosine A2B (human) receptor, in HEK-293 cells.

Product Variant
Unit Size: 400 units
Part #:
ES-013-M400UA
For research use only. Not for use in diagnostic procedures.
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Overview

Membrane Target Systems are quality assured frozen membranes from cells that express Adenosine A2B (human) recombinant or endogenous receptors.

We submit every batch of receptor to stringent quality controltesting that includes saturation radioligand binding assay todetermine receptor concentration (Bmax) and affinity (Kd).Competition binding assays are performed determine affinity (Ki)against known reference agonists and antagonists. GTPgS data isalso provided for some of our Gi coupled receptors.

Membranes are carefully prepared and ready for a variety of HTSapplications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtrationmethods).

Products are packaged as frozen crude membrane preparations. Oneassay unit is defined as micrograms of protein, defined bycompetition binding assay (filtration). A complete productdescription and recommended protocol are included on the ProductInformation Sheet.

Some of our receptors may be restricted for sale in specifiedcountries. Please inquire.

Specifications

Concentration
8 µg/µL protein
Volume
1000.0 µL
µg protein/unit
20 µg protein / unit
Application
Drug Discovery & Development
Brand
Membrane Target Systems
Buffer/Solvent
50 mM Tris-HCL (pH 7.4), 0.5mM EDTA, 10mM MgCl2, 10% sucrose
G-Alpha Natural Receptor
Gs
Host Cell Line
HEK293
Host Species
Human
Product Group
Membrane Preparation
Receptor
Adenosine
Shipping Conditions
Shipped in Dry Ice
Target Class
GPCR
Therapeutic Area
Cardiovascular
Central Nervous System
Inflammation
Unit Size
400 units
Validation
Binding

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