Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Revvity’s Edit-R™ from its Horizon Discovery product line predesigned synthetic sgRNAs result in high efficacy knockout of target genes as validated by western blot using Revvity’s Western Lightning™ ONE chemiluminescent substrates.
For research use only. Not for use in diagnostic procedures.
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules