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Application Note
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules
Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Revvity’s Edit-R™ from its Horizon Discovery product line predesigned synthetic sgRNAs result in high efficacy knockout of target genes as validated by western blot using Revvity’s Western Lightning™ ONE chemiluminescent substrates.
For research use only. Not for use in diagnostic procedures.
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Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules