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Technical Note

A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay

G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.

This technical note outlines a method for preparing GPCR membrane model extracts from stable cell lines, specifically for use with the HTRF GTP Gi binding assay.

Get this technical note and discover:

  • Key Highlights such as the Importance of GPCRs and the advantages of using HTRF GTP Gi Binding Assay
  • Detailed Method with Cell Culture Preparation, Cell Lysis, Membrane Preparation and Assay Optimization

For research use only. Not for use in diagnostic procedures.

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A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay

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