Case Study
A scalable method to monitor protein levels and localizations in cells
In this case study, we present a novel method developed by researchers at the CeMM Research Center for studying protein levels and localization within cells. Utilizing CRISPR-Cas9-based intron tagging, this scalable approach generates cell pools expressing hundreds of GFP-fusion proteins, enabling comprehensive analysis of cellular responses to perturbations. Through a combination of intron tagging with in situ sequencing, previously unrecognized proteins impacted by treatments are identified, surpassing the limitations of existing high-throughput methods. This approach can be applied to other sets of genes beyond metabolic enzymes and potentially in a genome-wide manner to study protein dynamics at scale.
For research use only. Not for use in diagnostic procedures.
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A scalable method to monitor protein levels and localizations in cells