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Technical Note

A scalable and reproducible workflow for high-content analysis of cytotoxic effects in RASTRUM 3D cell cultures

Explore our Technical Note unveiling a robust workflow for advanced cytotoxicity analysis in 3D cell cultures. This method addresses challenges in reproducibility, scalability, and imaging accuracy, enhancing drug testing accuracy and physiological relevance.

Key Features:

  • Reproducible 3D Models: Learn the precise creation of 3D matrix cultures using the RASTRUM platform, ensuring consistent dimensions independent of cell type or conditions.
  • Tailored Microenvironment: Tailor matrix stiffness and biofunctionalization for physiologically relevant cell models, significantly improving drug-induced cytotoxicity predictions.
  • Imaging Enhancement: Overcome signal attenuation and data volume challenges in 3D cell culture imaging. Utilize water immersion objectives, optical clearing, and precise positioning techniques for optimal high-content image acquisition.

For a comprehensive exploration of this innovative workflow and its applications in 3D cytotoxicity analysis, download the complete Technical Note.

For research use only. Not for use in diagnostic procedures.

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A scalable and reproducible workflow for high-content analysis of cytotoxic effects in RASTRUM 3D cell cultures

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