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Application Note

RNA extraction from human coronavirus: a comparative study highlighting bead mill vs. manual methods

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Reliable extraction of viral RNA from tissue samples is an important step in viral detection for research studies. The Omni Bead Ruptor Elite bead mill homogenizer helps reduce the time needed for initial homogenization steps upstream RNA kit extractions, allowing simultaneous processing of up to 24 samples in 30 seconds in this proof-of-concept study using viral cultures. The protocol using bead mill homogenization upstream of sample vortexing demonstrated increased efficiency over traditional vortexing methods alone. The Omni Bead Ruptor Elite bead mill homogenizer is recommended for its increased throughput, robust RNA yields, and decreased time required for extractions of viral RNA from multiple samples.

For research use only. Not for use in diagnostic procedures.

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RNA extraction from human coronavirus: a comparative study highlighting bead mill vs. manual methods