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Application Note

Kinetic Binding of Kinase inhibitors and determination of Kon, Koff rates

Benefit from HTRF technology to enhance your inhibitor characterizations

Nowadays, determinations of Kon, Koff, and the residence time of inhibitors have become key parameters in the measurement of drug efficacy. However, getting an accurate kinetic binding determination and reproducible results can be tricky. With this application note, discover how HTRF kinase binding platform assays can be used for kinetic binding experiments. It explains:

  • The materials and methods required
  • Data processing and result analyses
  • Concrete case studies

For research use only. Not for use in diagnostic procedures.

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Kinetic Binding of Kinase inhibitors and determination of Kon, Koff rates

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