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Application Note

High yield, automated DNA extraction from bovine liver utilizing bead mill homogenization

nucleic-acid-extraction-from-hepg2-512

The processing of human and animal tissue samples from experimental studies for downstream molecular analysis is a cornerstone of pre-clinical research across multiple disciplines spanning agrigenomics and disease research. Within animal anatomy, soft tissues are a vital component of not only organ-specific research, but also for providing insights into drug discovery or development work. Genomic workflows, including the critical upfront sample prep for efficient nucleic acid isolation of DNA and/or RNA is an important application area. Though there are multiple methods for tissue disaggregation and subsequent sample homogenization, bead mill homogenization is particularly effective for its ability to provide consistent and uniform homogenates via mechanical forces and cell disruption. In the same vein, the incorporation of automation into workflows reduces the risk of introducing human error and inconsistencies between samples. In this application note, an automation-enabled workflow for bead mill homogenization into an automated nucleic acid extractor is described to observe run-to-run reproducibility between the groups of samples extracted for DNA on different days using the same method.

For research use only. Not for use in diagnostic procedures.

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High yield, automated DNA extraction from bovine liver utilizing bead mill homogenization

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