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Application Note

High-throughput genomic DNA and protein extraction from multiple tissue types using the Omni Bead Ruptor 96 bead mill homogenizer

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In the field of pre-clinical and clinical research, sample sourcing and the realistic quantities of tissues or rare samples available for biological analysis can be a major challenge for researchers. Comprehensive studies typically rely on both genomic expression and proteomic profiling readouts to gain for meaningful insights into biological processes, disease progression, or a drug’s therapeutic value, amongst other scientific questions. Well-plate homogenization is an important tool for high-throughput, rapid tissue disruption via bead-milling in well-plates for downstream genomic and proteomic analysis. In this application note, DNA integrity and the protein repertoire assessed using LC-MS/MS techniques for bottom-up proteomic analysis is reviewed after homogenization from rat brain, heart, and small intestine using the same instrument setting for full sample disruption.

For research use only. Not for use in diagnostic procedures.

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High-throughput genomic DNA and protein extraction from multiple tissue types using the Omni Bead Ruptor 96 bead mill homogenizer

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