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Technical Note
Evaluating protein phosphorylation and cellular signaling cascades within the integrated stress response pathway
Protein phosphorylation, regulated by kinase and phosphatase cascades, plays a crucial role in cellular processes. Monitoring phosphorylation events in cellular models aids in studying compound activity and understanding mechanisms of action.
In this technical note, we showcase the effectiveness of AlphaLISA™ SureFire® Ultra™ assays in accurately measuring targeted phosphorylation events and total protein levels, with a focus on the eIF2α/ATF-4 pathway in cell-based experiments. Download the note to learn more!
For research use only. Not for use in diagnostic procedures.
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Evaluating protein phosphorylation and cellular signaling cascades within the integrated stress response pathway