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Application Note
Effectiveness of CRISPR-Cas9 using pools of Dharmacon™ Edit-R™ synthetic crRNAs in high-content analysis screening experiments
Learn about the effectiveness of CRISPR-Cas9 using pools of Dharmacon™ Edit-R™ synthetic crRNAs in high-content analysis screening experiments.
In this application note, we demonstrate that:
- Pooling crRNAs to the same gene target can provide a robust functional knockout in arrayed CRISPR screening experiments.
- crRNA pools can work successfully in phenotypic screening experiments which can significantly reduce the cost of an experiment.
For research use only. Not for use in diagnostic procedures.
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Effectiveness of CRISPR-Cas9 using pools of Dharmacon™ Edit-R™ synthetic crRNAs in high-content analysis screening experiments