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Application Note

Download your 3 application notes on kinase inhibitor selection for kinase binding assays

Challenge kinase binding assays and win with HTRF™ technology

The 3 application notes present a new HTRF kinase binding assay that combines the usage of a fluorescent inhibitor with a tagged kinase enzyme and its corresponding anti-tag-Europium labeled detection reagent.

These 3 application notes will provide you with:

  • Validation data for the 3 fluorescent tracers  Dasatinib-red, Staurosporine-red and Sunitinib-red
  • Guidance for the determination of inhibitor constants: Kd and Ki
  • Pharmacological characterization of known inhibitors and correlation with literature data
  • Relevant case studies to help you revolutionize your assays 

For research use only. Not for use in diagnostic procedures.

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Application note
HTRF kinase binding assays: Dasatinib-Red validation
This note presents a new HTRF kinase binding assay that combines the usage of a Dasatinib-Red ligand with a tagged kinase enzyme and its corresponding anti-tag-europium labeled detection reagents.
Learn more
Application note
HTRF kinase binding assays: Staurosporine-Red validation
This note presents a new HTRF kinase binding assay that combines the usage of a Staurosporine-Red ligand with a tagged kinase enzyme and its corresponding anti-tag-Europium labeled detection reagents.
Learn more
Application note
HTRF kinase binding assays: Sunitinib-Red validation
This note presents a new HTRF® kinase binding assay that combines the usage of a Sunitinib-Red ligand with a tagged kinase enzyme and its corresponding anti-tag- Europium labeled detection reagents.
Learn more