Nematodes, with their vast diversity, have adapted to various environments and include both parasitic and non-parasitic species. The vinegar eel, Turbatrix aceti, is a non-parasitic nematode used in DNA damage studies and is easy to maintain in unpasteurized vinegar. We demonstrate an efficient DNA extraction method from T. aceti using the Omni Bead Ruptor 4 bead mill homogenizer, which prepared high-integrity homogenates suitable for downstream DNA extraction. The optimizations help ensure minimal DNA shearing and consistent DNA concentration, highlighting the homogenizer’s effectiveness in sample preparation.
For research use only. Not for use in diagnostic procedures.
DNA extraction from Turbatrix aceti using the Omni Bead Ruptor 4 bead mill homogenizer