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Technical Note

Designing your own AlphaLISA assay: Selecting toolbox bead pairs to avoid potential bead-bead interactions

Optimizing AlphaLISA Assays: Bead Pairing Recommendations.

In AlphaLISA assay design, selecting the right toolbox beads is crucial. These beads impact assay performance and can make the difference between a successful assay and an unworkable one. This technical note provides recommendations on bead pairings, highlighting host species of antibodies on each toolbox bead, along with data generated in the absence of analyte and other assay components.

For research use only. Not for use in diagnostic procedures.

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Designing your own AlphaLISA assay: Selecting toolbox bead pairs to avoid potential bead-bead interactions