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Application Note

Controlling sample temperature during bead milling using passive cooling with the Omni Bead Ruptor Elite 7 mL tube carriage

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Bead mill sample homogenization, exemplified by the Omni Bead Ruptor Elite™ homogenizer, is a rapid and efficient method for sample disruption. However, for longer processing, the kinetic energy generated can increase sample temperature to potentially affect heat-sensitive analytes. We evaluated the passive cooling effect of the 7 mL carriage on larger volume samples, with the carriage and buffers pre-cooled to various temperatures. The results showed that using a carriage equilibrated at -80 °C in combination with chilled buffers effectively kept processing temperatures below 15 °C for up to 1 minute of processing time for larger volume samples though for samples that are in 2mL tubes, active chamber cooling is preferred utilizing a cryo cooling unit.

For research use only. Not for use in diagnostic procedures.

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Controlling sample temperature during bead milling using passive cooling with the Omni Bead Ruptor Elite 7 mL tube carriage

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