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Technical Note
Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants
Cytokine Detection Using AlphaLISA Biotin-Free assays
This technical note describes the use of AlphaLISA biotin-free kits for detecting and quantifying cytokines, including interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6), in supernatants produced by human PBMCs cultured for two days with or without Dynabead® stimulation.
For research use only. Not for use in diagnostic procedures.
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Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants