Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A

Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.

Therapeutic antibody engineering programs may aim to increase the affinity for certain FcγRs while decreasing the affinity for others. Robust, transferable assays are needed to determine the binding affinity for a particular therapeutic antibody to all of the FcγRs, to generate a ‘characterization binding profile’ that can help determine a therapeutic antibody’s MOA and potential off-target effects.

In this application note we demonstrate the ease with which AlphaLISA toolbox reagents can be used to develop any FcγR binding assay/ across the various stages of biologics research and development, including therapeutic screening and GMP Lot Release.

For research use only. Not for use in diagnostic procedures.