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Technical Note
AlphaLISA G9a Histone H3-Lysine N-methyltransferase assay
Quantification of Histone H3 Lysine 9 Di-Methylation Using AlphaLISA Assay
In this technical note discover how The AlphaLISA assay detects di-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 9. In this assay, a biotinylated histone H3-derived peptide serves as the substrate, and the modified substrate is detected using Streptavidin (SA) Alpha Donor beads and AlphaLISA Acceptor beads conjugated to an antibody specific to the epigenetic mark of interest.
For research use only. Not for use in diagnostic procedures.
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AlphaLISA G9a Histone H3-Lysine N-methyltransferase assay