Target Engagement Reagents

Discover Alpha SureFire ®   Ultra ™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology and sandwich immunoassays for detecting phosphorylated proteins in cells. Offering a quantitative alternative to Western Blotting, Alpha SureFire assays are automation-friendly, easily miniaturized, and proficient in detecting both endogenous and recombinant proteins. Explore our comprehensive portfolio of SureFire Assays, designed to help you elevate and expedite your drug discovery journey.

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are obtained.

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay Several biological processes are regulated by protein phosphorylation. It is, therefore, no surprise that the dysregulation of protein phosphorylation is implicated in a relatively large number of diseases. AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments. This guide contains tools and data helpful for you to perform your assays using AlphaLISA SureFire Ultra: A detailed description of the assay and its options A thorough investigation of assay conditions to obtain the optimal response from the chosen modulator and cell line A list of optimization steps to provide a sufficient assay window and produce the strongest results possible

Atherosclerosis pathogenesis, cellular actors, and pathways Atherosclerosis is a common condition in which arteries harden and become narrow due to a build-up of fatty material, usually cholesterol, and other substances such as calcium. This can lead to a range of serious health complications, including heart attack or stroke, making the disease an important contributing factor in death and morbidity in developed countries. Recent developments in our understanding of atherosclerosis from a molecular perspective include the discovery of new players in disease pathogenesis. Included in this white paper Atherosclerosis: step-by-step pathogenesis, therapeutic strategies, and recent developments Detailed descriptions and explanations, including a focus on pathways

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies and small molecules. CCR7 and CXCR2 receptors, which are expressed in immune cells and are therapeutic targets for disorders like lupus erythematosus, adult leukemia, lymphomas, chronic obstructive pulmonary disease (COPD), and sepsis. AlphaLISA ™ SureFire ® Ultra ™ and Alpha SureFire ® Ultra ™ Multiplex assays are automation-friendly, applicable to both small and large-scale screens, and can assess phosphorylation status in complex matrices. The LANCE Ultra cAMP assay is measures cyclic AMP (cAMP) produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs). This application note demonstrates how the SureFire Ultra and LANCE Ultra cAMP assays can be used for measuring inhibitors to CCR7 and CXCR2 cell surface receptors using a cellular model system where these receptors are overexpressed in CHO cells. The assays were optimized to measure receptor blockage and assayed receptor activity modulation by detecting ERK and AKT phosphorylation status and cAMP modulation. For more details, download the application note!

Emerging pathways to neuroinflammation and neurodegeneration Neurodegenerative diseases, such as amyotrophic lateral sclerosis, Parkinson's disease, or Alzheimer's disease, occur as a result of neuroinflammation and neurodegenerative progression. For the moment, these diseases are incurable. However, as research progresses, many similarities between these diseases have been found at a sub-cellular level. Review the fundamentals of the neuroinflammation process Learn from a cutting-edge research report Detailed insight into neurodegenerative diseases

Protein phosphorylation, regulated by kinase and phosphatase cascades, plays a crucial role in cellular processes. Monitoring phosphorylation events in cellular models aids in studying compound activity and understanding mechanisms of action. In this technical note, we showcase the effectiveness of AlphaLISA ™ SureFire ® Ultra ™ assays in accurately measuring targeted phosphorylation events and total protein levels, with a focus on the eIF2α/ATF-4 pathway in cell-based experiments. Download the note to learn more!

The cell cycle is tightly regulated by key proteins like Cyclin D1, which forms complexes to initiate progression through phases like G1 into S and G2. Dysregulation of these proteins, including Cyclin A2, Cyclin B1, Cyclin D1, and Cyclin E1, can serve as prognostic biomarkers in cancer, potentially driving uncontrolled proliferation and cancer progression. Discover insights on cell cycle regulation in this technical note using AlphaLISA ™ SureFire ® Ultra ™ immunoassay technology, investigating molecular responses to PROTAC and NRG1 treatments. Download the full document to learn more.

An in-depth review of molecular and cellular pathways The maintenance of proteostasis, the biological mechanisms that control the fate of a protein from synthesis to degradation, is ensured by complex and interconnected pathways controlling protein abundance, turnover, folding, functions, subcellular localization, and ultimate degradation. Impaired proteostasis can lead to the accumulation of damaged or aggregated proteins, or conversely, to excessive protein degradation. Proteostasis is thus involved in pathologies such as cancer, autoimmune diseases, and neurodegenerative disorders. This protein degradation booklet helps scientists and researchers appreciate and navigate the diversity of molecular pathways associated with protein degradation. Check out this exclusive guide that includes: Key information about the ubiquitin structure, function, and regulation The cellular and molecular understandings on proteasome and autophagy degradation machinery An overview of current therapeutic strategies, as well as the promising PROTAC approach to specifically target protein degradation

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

Neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and Huntington’s, are complex disorders that affect millions worldwide. Traditional treatments focus on managing symptoms, but recent advancements in gene therapy offer hope for more effective and lasting solutions. Key insights: Understanding neurodegenerative diseases: Learn about the prevalence, impact, and current treatment limitations neurodegenerative diseases. Gene therapy mechanisms: Explore how gene therapy works, including CRISPR gene editing, RNA interference, and other cutting-edge techniques. Approved therapies: Get detailed information on the four FDA-approved gene therapies for neurodegenerative diseases, including their mechanisms and delivery methods. Future directions: Discover the latest advancements in gene editing technologies and delivery systems that are paving the way for more effective treatments. Why Gene Therapy? Gene therapy represents a revolutionary approach to treating neurodegenerative diseases by targeting the root causes at the genetic level. This white paper delves into the science behind these therapies and their potential to transform patient outcomes. Exclusive Content: Detailed explanations of genome and RNA editing techniques. Insights into the latest delivery methods for gene therapy. Case studies of approved gene therapies and their impact. Future trends and research directions in the field. Fill out the form below to access the complete white paper and stay updated with the latest research and developments. For research use only. Not for use in diagnostic procedures.

Human fibrotic diseases are a significant health problem worldwide due to the large number of people affected and the incomplete knowledge of the fibrotic process’s pathogenesis. Advance your research on fibrosis and benefit from Revvity’s broad offering of reagent technologies. For research use only. Not for use in diagnostic procedures.

This document includes detailed tables listing HTRF™, AlphaLISA™ SureFire® Ultra™, and Alpha SureFire® Ultra™ Multiplex assays. Each assay is evaluated for its compatibility with species including Human, Mouse, Rat, Monkey, Hamster, Drosophila, Pig, and Dog. For research use only. Not for use in diagnostic procedures.

Obesity is a complex condition characterized by excessive fat accumulation, posing significant health and socioeconomic challenges globally. Our in-depth guide delves into the intricate cellular processes contributing to obesity, offering insights into potential therapeutic avenues. Main cellular mechanisms included in this guide: Adipogenesis: understand the formation of fat-storing cells, key transcription factors, and intracellular signaling pathways. Lipid metabolism: learn about the processes involved in lipid digestion, absorption, transportation, storage, and degradation. Insulin signaling: discover the role of insulin in metabolism and the impact of insulin resistance. Neuroendocrine regulation of appetite: explore how hormones and neurotransmitters control appetite and food intake. For research use only. Not for use in diagnostic procedures.