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Fluorescent secondary antibodies are everyday tools for researchers performing high-content analysis, cellular imaging, flow cytometry, and western blotting. The method of indirect immunofluorescence detection relies on an unconjugated antigen-specific primary antibody coupled with a fluorescent secondary antibody.
Secondary fluorescent antibodies offer enhanced sensitivity – binding to the primary antibody and then amplifying the fluorescence signal. In addition, they provide greater flexibility since they can be easily combined for multiplexing experiments.
Our range of extensively validated PhenoVue™ fluor-labeled secondary antibodies features:
For research use only. Not for use in diagnostic procedures.