Skip to main content

Low-pass WGS and Imputation Analysis

Revvity with its partners offers a complete solution for genotyping based on low-pass whole genome sequencing (LP-WGS) followed by imputation. This approach involves sequencing the entire genome at coverages in the range of 0.5x to 5x combined with imputation analysis to infer missing genotypes. LP-WGS has emerged as a popular alternative to microarrays or high-depth sequencing, particularly valuable for plant and animal breeders where cost considerations are crucial.

  LP-WGS WGS Arrays
Coverage Low Medium N/A
Relative Expense Low High Low
Novel SNP Detection
CNV Detection Limited

 

With expertise marrying automation and reagents, we are uniquely positioned to deliver broad, single source systems that offer the flexibility needed to meet today’s changing demands.

  • Sample homogenization
  • Nucleic acid extraction
  • QC and analysis solutions
  • Library preparation reagents
  • Modular and scalable automation
  • Imputation analysis

Molecular Genotyping

Improving genetics for traits like higher yield, drought tolerance, and disease susceptibility relies on translating genome wide information into screening and prediction tools for efficient breeding. A few years back microarray technology allowed researchers to analyse thousands of genetic markers simultaneously, providing valuable insights into traits of agricultural importance. Microarrays played a crucial role in identifying quantitative trait loci and developing marker-assisted selection strategies for crop improvement.

However, microarrays had limitations, including the need for prior knowledge of genetic sequences, only produce a small fraction of genomic information, and have little ability to detect novel variants.

The arrival of NGS combined with robust imputation pipelines marked a paradigm shift in agrigenomics. This breakthrough opened new avenues for exploring genetic diversity, discovering novel traits, and accelerating breeding programs without the complexities of variant selection, probe design and call training associated with developing SNP arrays.
 

For research use only. Not for use in diagnostic procedures.

Low-pass WGS and Imputation Analysis

Automation-enabled sample homogenization

For genomic applications, multiple factors need to be considered and optimized to ensure that the nucleic acid going into genomic assays will maintain enough integrity to produce meaningful results. Within these factors, automation could add reliability and consistency in upfront sample homogenization that leads to more efficient nucleic acid extraction of usable DNA or RNA. Sample homogenization via automated rotor-stator or bead-mill technologies can significantly increase workflow efficiency and operation costs associated with human resources or discrepancies in results between replicate QC or cross-site method optimization.
 

omni-br-elite.png

Nucleic acid isolation from agricultural samples

Fast, cost-effective nucleic acid extraction from flexible input volumes

With flexibility in throughput, capacity, and sample type, robust manufacturing standards, and outstanding customer support, chemagic™ nucleic acid extraction automation solutions process sample volumes ranging from 50 µL to 18 mL cost effectively and quickly.

Learn more

Nucleic acid analysis for agrigenomics

Improve your nucleic acid analysis with the automated LabChip® solutions and enable high throughput analysis of genomic DNA, RNA, and NGS libraries in seconds.

Reporting is simplified through the automatic generation of data as an electropherogram, virtual gel, or tabular report.

Learn more

DNA library prep for low pass sequencing applications

The NEXTFLEX HT Agrigenomics Low-Pass WGS kit is designed for the highly efficient conversion of DNA, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO.

Learn more

Modular and scalable automation

modular-and-scalable-automation


Our automation solutions allow labs performing agricultural research to standardize procedures and implement best practices increasing throughput, decreasing costs, and ensuring sample integrity and security. Flexible modules offer scalability in throughput, capacity and dynamic volume range, high quality manufacturing standards, and outstanding customer service and support.

Increasing throughput and sample security

Modular, scalable solutions address sample security requirements and changing throughput and workflow requirements to automate entire agricultural genomics workflows.

Single source integratio

explorer™ G3 workstations seamlessly integrate many of the critical agrigenomics processes across platforms. With integrated nucleic acid isolation, sample assessment and a modular approach for increasing throughput, a custom explorer G3 workstation can be designed to align with any analytical pipeline inputs.

Learn more

ご質問がございましたら、
お気軽にお問い合わせください。

お問合せ