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      Revvity Impact Report

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  • Products
    • 研究・開発
      • ゲノム解析
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGSワークフロー
        • Microplate Readers
        • マイクロ流体核酸分析
        • CRISPR Technologies
        • RNA Interference
        • トランスフェクション試薬および補助試薬
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
        • Single-Cell Sequencing
        • Labeled Nucleotides
        • qPCR
        • ライブラリー調製キット
        • Magnetic Beads
        • Viral Vector Products
        Revvity Impact Report

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      • タンパク質解析
        • Binding Assays
        • イムノアッセイ
        • マイクロ流体によるタンパク質特性評価
        • Microplate Readers
        • マイクロプレート
        • Recombinant Proteins
        • ウェスタンブロッティング
        • Sample Dissociation
        • M-PVA Magnetic Beads
        Revvity Impact Report

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      • 細胞分析
        • Cell Isolation
        • Cell Lines & Stem Cells
        • Cell Counting and Image Cytometry
        • セルヘルスと細胞生存率
        • Cellular Imaging & Analysis
        • Immunoassays
        • In Vivo Imaging
        • Microplates
        Revvity Impact Report

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      • 研究ソリューション
        • Immunoassays Solutions
        • pHSense Reagents
        • Biomarker Discovery
        • 細胞・遺伝子治療
        • GMP Workflows
        • バイオロジクス
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        • プレシジョン・メディシン・リサーチ
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        • アッセイ開発ワークフロー
        • Physiological Model Solutions
        • Biobanking Workflows
        • Agrigenomics Workflows
        Revvity Impact Report

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        Impact Report

      Revvity Impact Report

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      Impact Report

    • 臨床・診断
      • リプロダクティブ・ヘルス
        • 出生前検査
        • 新生児スクリーニング
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • cell-free DNA解析
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • 分子細胞遺伝学
      • 感染症
        • 結核マネジメント
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
      • がん
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
      • 自己免疫(powered by Euroimmun)
        • EUROIMMUN Solutions
        • IDS Solutions
      • 内分泌学
      • アレルギー
      • 神経変性
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
      • ラピッド検査
    • Reagents
      • BioLegend
        Revvity Impact Report

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        Impact Report

      • Cellular Imaging Reagents
        Revvity Impact Report

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        Impact Report

      • chemagic Nucleic Acid Purification Kits
        Revvity Impact Report

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        Impact Report

      • CRISPR Technologies
        Revvity Impact Report

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        Impact Report

      • GPCR Research Reagents
        Revvity Impact Report

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        Impact Report

      • Immunoassays
        Revvity Impact Report

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        Impact Report

      • In Vivo Imaging Reagents
        Revvity Impact Report

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        Impact Report

      • Liquid Scintillation Cocktails
        Revvity Impact Report

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        Impact Report

      • NGS Library Prep Kits
        Revvity Impact Report

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        Impact Report

      • pHSense Reagents
        Revvity Impact Report

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        Impact Report

      • qPCR
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        Impact Report

      • Radiochemicals
        Revvity Impact Report

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        Impact Report

      • RNA Interference
        Revvity Impact Report

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        Impact Report

      • Primary and Secondary Antibodies
        Revvity Impact Report

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        Impact Report

      Revvity Impact Report

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      Impact Report

    • プラットフォームと自動化
      • 核酸分離
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
      • 自動液体分注
        • リキッドハンドリングワークステーション
        • 自動液体分注アプリケーション
        • 液体分注機消耗品&アクセサリー
      • 統合ラボオートメーション
        • 自動プレートローディング
        • ワークステーションモジュール
        • スケジューリング&コントロールソフトウェア
        • ロボット工学
      • マイクロ流体分析
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
      • 検出システムソリューション
        • Plate Reader Accessories
        • マイクロプレートリーダー
        • 放射性物質の検出
        • 放射性化学物質
        • 液体シンチレーションカクテル
        • 放射線測定用消耗品・アクセサリー
      • イメージング
        • Cellular Imaging & Analysis
        • In vivo イメージング
      • Cell Counting and Image Cytometry
      • サンプルのホモジナイゼーション
        • ホモジナイザー
        • ホモジナイザーアクセサリー
        • ホモジナイザー消耗品
        • サンプルの遠心分離
        • サンプルの超音波処理
      • IVDプラットフォーム&オートメーション
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
      • 消耗品&アクセサリー
        • Cassettes
        • Cell Harvesters
        • In Vivo Imaging Accessories
        • ハイコンテントイメージングアクセサリー
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
        • Transfer Membranes
        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
      • Instrument Service & Maintenance
        • On Demand Equipment Service
        • Equipment Service Plans
    • Consumables & Accessories
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    • Signals ソフトウエア
      • All Products
        • Signals One
        • ChemDraw
        • Spotfire®
        • Signals Clinical
        • Signals Notebook
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      • All Solutions
        • Biology
        • Discovery Chemistry
        • Clinical & Translational
        • Specialty Chemicals
        • Food/Flavor/Fragrance
        Revvity Impact Report

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        Impact Report

      Revvity Impact Report

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      Impact Report

    • Revvity Omics Services
      Revvity Impact Report

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      Impact Report

    Revvity Impact Report

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    Impact Report

  • Services
    • Preclinicalサービス
      • Antibody Drug Conjugate Services
      • Complex Cell Model Screening Services
      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
      • Immune Cell Screening
      • Functional Genomic Screening Services
      • Cell Panel Screening
      • Cell Line Engineering
        • CHOSOURCE CLE Services
      • Viral Vector Engineering and Manufacture
    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • 臨床サービス
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • ライセンシング
      • Gene Delivery Licensing
      • Gene Expression Systems
        • CHOSOURCE Platform
      • Pin-point™ Base Editing Platform
      • Virus Screening
    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM ソリューション
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

    Learn more

  • 会社
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  • Brands
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    • TotalSeq

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  • ホーム
  • Products
  • 研究・開発
  • タンパク質解析
    • ゲノム解析
    • タンパク質解析
    • 細胞分析
    • 研究ソリューション
  • イムノアッセイ
    • Binding Assays
    • イムノアッセイ
    • マイクロ流体によるタンパク質特性評価
    • Microplate Readers
    • マイクロプレート
    • Recombinant Proteins
    • ウェスタンブロッティング
    • Sample Dissociation
    • M-PVA Magnetic Beads
  • Cell Signaling Research Reagents
    • Autoimmune Disease Research Reagents
    • MASLD, MASH, and Fibrosis Research Reagents
    • Cell Signaling Research Reagents
    • Biologics Screening and Characterization Assays
    • Cell and Gene Therapy Research Reagents
    • Obesity, Diabetes and Metabolic Disease Reagents
    • Newest assay kits
    • Reagents for Deciphering the Interactome
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    • GPCR research reagents
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    • Target engagement reagents
    • Targeted Protein Degradation
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    • Oncology Drug Discovery Reagents

Cell Signaling Research Reagents

Intracellular protein detection is used to study signaling pathways, also known as signal transduction cascades, and plays a key role in sensing and integrating diverse external stimuli to generate biological responses. These stimuli are detected by cell surface receptors, which transmit the initial signal inside the cell. This signal is then amplified by a transduction cascade mediated by the phosphorylation of multiple protein substrates.

Protein phosphorylation is a dynamic process, tightly controlled by kinase and phosphatase cascades, which regulate many cellular processes and have been identified as underlying mechanisms for a variety of diseases.

The flexibility and robustness of HTRF™ and AlphaLISA™ SureFire® Ultra™ technologies make them ideal choices for researchers focused on cell signaling and intracellular phosphorylated proteins, offering a streamlined approach to pathway analysis.

HTRF
HTRF assay principle
Read guide Explore portfolio
AlphaLISA SureFire Ultra, Multiplex SureFire Ultra and AlphaLISA SureFire Biotin Free
Alpha assay principle
Read guide Explore portfolio

HTRF for pathway analysis

Intracellular signaling pathways, or signal transduction cascades, are essential for cells to sense and respond to various external signals like growth factors, hormones, cytokines, and stress. These pathways trigger biological responses such as enzyme activity changes, gene expression, and cytoskeletal adjustments, leading to critical cellular functions including proliferation, migration, differentiation, and nutrient metabolism.

HTRF cell-based phosphorylated and non-phosphorylated protein level immunoassay kits offer a rapid and streamlined alternative to traditional technologies such as Western blot, ELISA, and other luminescent bead-based assays.

Following treatment, cells undergo lysis, releasing intracellular proteins, both phosphorylated and unphosphorylated, from the cell compartments. The released proteins are then detected by adding the appropriate reagents.

All HTRF phospho and total assays are based on a sandwich immunoassay format

The HTRF phosphorylated protein immunoassay uses two antibodies: one labeled with a donor fluorophore and the other with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, while the second antibody recognizes the protein independently of its phosphorylation state.

The HTRF total protein immunoassay also uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for distinct epitopes on the protein.

HTRF total protein immunoassay also uses two labeled antibodies

Due to the sandwich format of HTRF intracellular phosphorylated and total assays, the HTRF signals increase proportionally to the concentration of the protein of interest.

HTRF signals increase proportionally to the concentration of the protein of interest

The proper optimization of assay conditions is essential to achieve the best reagent use and performance. Our guide offers the latest guidelines for optimizing cell signaling assays, ensuring you stay at the forefront of scientific research. With over 300 kits available, you will find the perfect solution for your specific needs.

Suggested content

Key guidelines to successful cell signaling experiments

AlphaLISA SureFire Ultra, Multiplex SureFire Ultra and AlphaLISA SureFire Biotin Free

AlphaLISA SureFire Ultra for quantifying phosphorylation events in cellular assays

AlphaLISA SureFire Ultra assay kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells. The AlphaLISA SureFire Ultra assay kits are optimized for enhanced signal-to-noise windows.

AlphaLISA assays require two bead types: Donor beads and Acceptor beads. In AlphaLISA SureFire Ultra, the Donor bead is coated with streptavidin to capture the biotinylated antibody while the Acceptor bead is coated with a proprietary "CaptSure™" agent that immobilizes the other antibody which is labeled with a CaptSure tag.

In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads in close proximity, whereby the singlet oxygen transfers energy to excite the Acceptor bead, enabling the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the quantity of phosphoprotein present in the sample.

Alpha SureFire Ultra assay principle

In addition to AlphaLISA SureFire Ultra kits detecting phosphorylated proteins, there is a subset of AlphaLISA SureFire Ultra kits that allow for the detection of the "total" analyte (i.e. non-phosphorylated as well as phosphorylated), offering an ideal way to normalize for positive or negative modulation of protein expression in cellular contexts.

Simultaneous detection of phosphorylated and non-phosphorylated with Multiplex assays

Multiplex SureFire Ultra (MPSU) kits are complete kits for phospho and total detection of the same target. Assessing the total protein level (phosphorylated and non-phosphorylated) is essential in distinguishing whether up- or down-regulation of phosphorylation is due to changes in that protein's expression levels or to cell viability.

Simultaneous detection of phosphorylated and non-phosphorylated with Multiplex assays

AlphaLISA SureFire Biotin Free: optimized for biotin-rich sample analysis

AlphaLISA SureFire Biotin Free platform is characterized by the incorporation of a different coating on the Alpha Donor Bead. We have introduced another proprietary "CaptSure 3" coating on the Alpha Donor Bead to replace the streptavidin coating that has long been associated with legacy Alpha technology.

AlphaLISA SureFire Biotin Free eliminates the need to consider free biotin in cell culture media, such as RPMI 1640, while maintaining high performance.

AlphaLISA SureFire Biotin Free: Optimized for Biotin-Rich Sample Analysis

Suggested content

Key guidelines to successful AlphaLISA SureFire Ultra experiments

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