Cell Cycle Assay Kits
Cell Cycle Assay Kits for Cellometer™ cell counters and Celigo™ image cytometer
Cell cycle analysis is used to determine the proportion of cells in each stage of the cell cycle for a given cell population based on variations in DNA content. In our ViaStain cell cycle assays, each cell is stained with propidium iodide (PI), a fluorescent nuclear staining dye that intercalates with DNA. Because the dye cannot enter live cells, the cells are first fixed (with ethanol or methanol), and then stained with the cell cycle reagent. Cells preparing for division will contain increasing amounts of DNA and display proportionally increased fluorescence. Differences in fluorescence intensity are used to determine the percentage of cells in each phase of the cell cycle. We provide instrument-specific cell cycle assay kits for Cellometer cell counters and the Celigo image cytometer.
For research use only. Not for use in diagnostic procedures.
The histogram shows fluorescent intensity of Jurkat cells at various stages of the cell cycle. The purple bar (2n) represents G1-phase , the gray bar S-phase, and teal bar (4n) the G2/M phase.
