DELFIA Citations

Overview

On this page we provide selected citations for each application.

Biodistribution

Journal articles

1. Bugelski, P.J. et al. CNTO 530: Molecular Pharmacology in Human UT-7EPO Cells and Pharmacokinetics and Pharmacodynamics in Mice. J Biotechnol 134, 171-180 (2008).
2. DeFrees, S. et al. GlycoPEGylation of Recombinant Therapeutic Protein Produced in Eschericia coli. Glycobiology 16, 833-843 (2006).
3. Ivey, N.S., Martin, J., Scheld, W.M., & Nathan, B.R. A New Method for Measuring Blood-Brain Barrier Permeability Demonstrated with Europium-Bound Albumin During Experimental Lipopolysaccharide (LPS) Induced Meningitis in the Rat. J of Neurosci Meth 142, 91-95 (2005).
4. Hale, S.L., Daí, W., Dow, J.S., Kloner, R.A. Mesenchymal Stem Cell Administration at Coronary Artery Reperfusion in the Rat by Two Delivery Routes: A Quantitative Assessment. Life Sci 83, 511-515 (2008).
5. Mignet, N. et al. Liposome Biodistribution by Time Resolved Fluorimetry of Lipophilic Europium Complexes. Eur Biophys J 35, 155-161 (2006).
6. Neville, M.E. et al. A Comparison of Biodistribution of Liposomal and Soluble IL-2 by a New Method Based on Time-Resolved Fluorometry of Europium. Cytokine 12, 1702-1711 (2000).

Biomolecular interaction

(protein-protein interaction, protein-peptide interaction, etc.) 
 

Journal articles

1. Sato, A., Kumagai, T., Aoki, J. & Ebina, K. Synthetic biotinylated peptide compounds derived from Asp-hemolysin: Novel potent inhibitors of platelet-activating factor. European journal of pharmacology (2012).
2. Kwok, W. W. et al. Frequency of Epie-Specific Naive CD4+ T Cells Correlates with Immunodominance in the Human Memory Repertoire. Journal of Immunology (Baltimore, Md.: 1950) (2012)
3. De, S.K. et al. Design, Synthesis, and Structure−Activity Relationship of Substrate Competitive, Selective, and in Vivo Active Triazole and Thiadiazole Inhibitors of the c-Jun N-Terminal Kinase. J Med Chem 52, 1943-1952 (2009).
4. Delerive, P. et al. PGC-1 Functions as a Transcriptional Coactivator for the Retinoid X Receptors. J Biol Chem 277, 3913-3917 (2002).
5. Glickman, J.F. et al. A Comparison of ALPHAScreen, TR-FRET, and TRF as Assay Methods for FXR Nuclear Receptors. J Biomol Screen 7, 3-10 (2002).
6. Kadkhodayan, S. et al. Evaluation of Assay Technologies for the Identification of Protein-Peptide Interaction Antagonists. Assay Drug Dev Technol 5, 501-513 (2007).
7. Rega, M.F., Reed, J.C. & Pellecchia, M. Robust Lanthanide-Based Assays for the Detection of Anti-Apoptotic Bcl-2-Family Protein Antagonists. Bioorg Chem 35, 113-120 (2007).
8. Stebbins, J.L. et al. Identification of a New JNK Inhibitor Targeting the JNK-JIP Interaction Site. Proc Natl Acad Sci U S A. 105, 16809--16813 (2008).

Revvity technical publications

1. Hurskainen, P., Frang, H., Vikstrom, S., Karvinen, J. & Hemmila, I. Heterogeneous and Homogeneous Time-resolved Fluorescence-Based Assays for a Low-Affinity Binding Reaction. Revvity Poster.

Cell cytotoxicity kit

Journal articles

1. Murakami, Y. et al. Toso, a functional IgM receptor, is regulated by IL-2 in T and NK cells. J. Immunol. 189, 587–597 (2012).
2. Chen, H.-M. et al. Shikonin induces immunogenic cell death in tumor cells and enhances dendritic cell-based cancer vaccine. Cancer immunology, immunotherapy: CII (2012).
3. Miller, A. S., Tejada, M. L. & Gazzano-Santoro, H. Development of an ELISA based bridging assay as a surrogate measure of ADCC. Journal of Immunological Methods (2012).
4. Amann, M. et al. Therapeutic Window of an EpCAM/CD3-Specific BiTE Antibody in Mice is Determined by a Subpopulation of EpCAM-Expressing Lymphocytes that is Absent in Humans. Cancer Immunol Immun 58, 95-109 (2009).
5. Conaghan, P. et al. Targeted Killing of Colorectal Cancer Cell Lines by a Humanised IgG1 Monoclonal Antibody that Binds to Membrane-Bound Carcinoembryonic Antigen. Br J Cancer 98, 1217-1225 (2008).
6. Poggi, A. et al. Effective in Vivo Induction of NKG2D Ligands in Acute Myeloid Leukaemias by All-Trans-Retinoic Acid or Sodium Valproate. Leukemia 23, 641-648 (2009).
7. Weber, G. et al. WT1 Peptide-Specific T Cells Generated from Peripheral Blood of Healthy Donors: Possible Implications for Adoptive Immunotherapy After Allogeneic Stem Cell Transplantation. Leukemia 23, 1634-1642 (2009).

Revvity technical publications

1. Sippola, K. et al. Cell Based Assay for Studying the Effects of Cytotoxic Agents. Revvity Poster. 
2. Vikstrom, S., Lahde, T. & Blomberg, K. Technical Tips for the Cell Cytotoxicity Assay using the DELFIA Technology. Revvity Application Note. 
3. A new simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays. Revvity Application Note.

Cell proliferation kit

Journal articles

1. Wu, T. et al. Immunosuppressive drugs on inducing Ag-specific CD4(+)CD25(+)Foxp3(+) Treg cells during immune response in vivo. Transpl. Immunol. 27, 30–38 (2012).
2. Malaguarnera, R. et al. Proinsulin Binds with High Affinity the Insulin Receptor Isoform A and Predominantly Activates the Mitogenic Pathway. Endocrinology (2012).Brillard, E. et al. Natural Killer Cells Prevent CD28-Mediated Foxp3 Transcription in CD4+CD25- T Lymphocytes. Exp Hematol 35, 416-425 (2007).
3. Brunati, A.M. et al. Cross-Talk Between PDGF and S1P Signalling Elucidates the Inhibitory Effect and Potential Antifibrotic Action of the Immunomodulator FTY720 in Activated HSC-Cultures. Biochimica Biophysica Acta 1783, 347-359 (2008).
4. Hoo, W.S. et al. Vaccination with Cell Immunoglobulin Mucin-1 Antibodies and Inactivated Influenza Enhances Vaccine-Specific Lymphocyte Proliferation, Interferon-γ Production and Cross-Strain Reactivity. Clin Exp Immunol 145, 123--129 (2006).
5. Koslowski, M. et al. MS4A12 Is a Colon-Selective Store-Operated Calcium Channel Promoting Malignant Cell Processes. Cancer Res 68, 3458-3466 (2008).
6. Liu, Z. & Habener, J.F. Glucagon-Like Peptide-1 Activation of TCF7L2-Dependent Wnt Signaling Enhances Pancreatic Beta Cell Proliferation. J Biol Chem 283, 8723-8735 (2008).
7. Pallandre, J. et al. Role of STAT3 in CD4+CD25+FOXP3+ Regulatory Lymphocyte Generation: Implications in Graft-versus-Host Disease and Antitumor Immunity. J Immunol 179, 7593-7604 (2007).
8. Selmani, Z. et al. Human Leukocyte Antigen-G5 Secretion by Human Mesenchymal Stem Cells Is Required to Suppress T Lymphocyte and Natural Killer Function and to Induce CD4+CD25highFOXP3+ Regulatory T Cells. Stem Cells 26, 212-222 (2008).
9. Villa, A.M. et al. Choline and Phosphatidylcholine Fluorescent Derivatives Localization in Varcinoma Vells Dtudied by Laser Scanning Confocal Fluorescence Microscopy. Eur J Cancer 41, 1453-1459 (2005).

Revvity technical publications

1. Vikstrom, S., Pulli, P., Hurskainen, P. & Gripenberg-Lerche, C. Multiplexing of DELFIA Cell Proliferation and DNA Fragmentation Assays with Conventional Fluorometric Cell-Based Assays for Identification of Toxic Compounds. Revvity Poster. 
2. DELFIA Cell Proliferation Kit: Optimized protocol for fixation of suspension cells. Revvity Application Note. 

Immunoassay/ELISA conversion

Journal articles

References using capture antibody

1. Hu, Z. et al. Detection of hepatitis B virus PreS1 antigen using a time-resolved fluoroimmunoassay. J Immunoassay Immunochem 33, 156–165 (2012).
2. May, R. et al. Preclinical development of CAT-354, an IL-13-neutralising antibody, for the treatment of severe uncontrolled asthma. British Journal of Pharmacology (2011).
3. Haneda, S. et al. Interleukin-1 Receptor Antagonist Expression in the Equine Endometrium During the Peri-Implantation Period. Domest Anim Endocrin 36, 209-218 (2009).
4. Hemmilä, I., Holttinen, S., Pettersson, K. & Lövgren, T. Double-Label Time-Resolved Immunofluorometry of Lutropin and Follitropin in Serum. Clin Chem 33, 2281-2283 (1987).
5. Peruski, A.H., Johnson, L.H. & Peruski, L.F. Rapid and Sensitive Detection of Biological Warfare Agents Using Time-Resolved Fluorescence Assays. J Immunol Methods 263, 35-41 (2002).
6. Smith, D.R. et al. Comparison of Dissociation-Enhanced Lanthanide Fluorescent Immunoassays to Enzyme-Linked Immunosorbent Assays for Detection of Staphylococcal Enterotoxin B, Yersinia pestis-Specific F1 Antigen, and Venezuelan Equine Encephalitis Virus. Clin Diagn Lab Immun 8, 1070-1075 (2001).
7. Zhou, F., Goodsell, A., Uematsu, Y. & Vajdy, M. Prolonged Protection Against Intra-Nasal Challenge with Influenza Virus Following Systemic or Combinations of Mucosal and Systemic Immunizations with a Heat Labile Toxin Mutant. Clin Vaccine Immunol 16, 471-478 (2009).

References using capture antigen

1. Lai, R. P. J. et al. Mixed Adjuvant Formulations Reveal a New Combination That Elicit Antibody Response Comparable to Freund’s Adjuvants. PLoS ONE 7, e35083 (2012).
2. Aggerbeck, H., Gizurarson, S., Wantzin, J. & Heron, I. Intranasal Booster Vaccination Against Diphtheria and Tetanus in Man. Vaccine 15, 307-316 (1997).
3. Atti, M.L.C.D. et al. Reactogenicity and Immunogenicity of Adult Versus Paediatric Diphtheria and Tetanus Booster Dose at 6 Years of Age. Vaccine 20, 74-79 (2001).
4. Hunolstein, C.V. et al. European Sero-Epidemiology Network: Standardisation of the Results of Diphtheria Antitoxin Assays. Vaccine 18, 3287-3296 (2000).
5. Maple, P.A.C. et al. Performance of a Time-Resolved Fluorescence Immunoassay for Measuring Varicella-Zoster Virus Immunoglobulin G Levels in Adults and Comparison with Commercial Enzyme Immunoassays and Merck Glycoprotein Enzyme Immunoassay. Clin Vaccine Immunol 13, 214-218 (2006).
6. von Hunolstein, C. et al. Diphtheria Antibody Levels in the Italian Population. Eur J Clin Microbiol 19, 433-437 (2000).

Whole cell-based

1. Hardcastle, A. et al. A Duplexed Phenotypic Screen for the Simultaneous Detection of Inhibitors of the Molecular Chaperone Heat Shock Protein 90 and Modulators of Cellular Acetylation. Mol Cancer Ther 6, 1112-1122 (2007).
2. McEarchern, J.A. et al. Preclinical Characterization of SGN-70, a Humanized Antibody Directed Against CD70. Clin Cancer Res 14, 7763-7772 (2008).
3. Zerwes, H. et al. A Multiparameter Screening Assay to Assess the Cytokine-Induced Expression of Endothelial Cell Adhesion Molecules. Anal Biochem 304, 166-173 (2002).

Revvity technical publications

1. DELFIA Immunoassays. Revvity Application Note. 
2. How to optimize rapid and simple immunoassays. Revvity Application Note. 
3. Vikstrom, S., Gripenberg-Lerche, C., Hurskainen, P. & Hemmilä, I. A Cell-based DELFIA Assay for Measuring Phosphorylation of Proteins. Revvity Application Note. 

Kinase

Journal articles

1. Newbatt, Y. et al. Identification of Auhosphorylation Inhibitors of the Inositol-Requiring Enzyme 1 Alpha (IRE1α) by High-Throughput Screening Using a DELFIA Assay. J Biomol Screen (2012).
2. Koziolek, E. J. et al. A high-affinity ErbB4Fc fusion protein is a potent antagonist of heregulin-mediated receptor activation. Growth Factors (2012).
3. Walton, M. I. et al. CCT244747 is a novel, potent and selective CHK1 inhibitor with oral efficacy alone and in combination with genotoxic anticancer drugs. Clin. Cancer Res. (2012).
4. Liu, Y. et al. Interacting Neuroendocrine and Innate and Acquired Immune Pathways Regulate Neutrophil Mobilization from Bone Marrow following Hemorrhagic Shock. J Immunol 182, 572-580 (2009).
5. Toral-Barza, L. et al. Characterization of the Cloned Full-Length and a Truncated Human Target of Rapamycin: Activity, Specificity, and Enzyme Inhibition as Studied by a High Capacity Assay. Biochem Bioph Res Co 332, 304-310 (2005).
6. Yamamoto, N. et al. Development of a High-Throughput Fluoroimmunoassay for Syk Kinase and Syk Kinase Inhibitors. Anal Biochem 315, 256-261 (2003).
7. Zhang, N. et al. MEK (MAPKK) Inhibitors. Part 2: Structure--Activity Relationships of 4-Anilino-3-Cyano-6,7-Dialkoxyquinolines. Bioorg Med Chem Lett 11, 1407-1410 (2001).

Revvity technical publications

1. Tamminem, J. et al. Screening for Specific Antibodies for Ser/Thr Kinase Assays Based on Time-Resolved Fluorescence. Revvity Poster. 
2. Vikstrom, S., Gripenberg-Lerche, C., Hurskainen, P. & Hemmilä, I. A Cell-based DELFIA Assay for Measuring Phosphorylation of Proteins. Revvity Application Note. 
3. Vikstrom, S., Korte, M., Pulli, P., Hurskainen, P. & Gripenberg-Lerche, C. A Cell-Based, Microplate Format, DELFIA Assay for Determination of the Activation of MAP Kinase. Revvity Poster. 

Multiplexing

Journal articles

1. Aggerbeck, H., Nørgaard-Pedersen, B. & Heron, I. Simultaneous Quantitation of Diphtheria and Tetanus Antibodies by Double Antigen, Time-Resolved Fluorescence Immunoassay. J Immunol Methods 190, 171-183 (1996).
2. Bookout, J.T. et al. Development of a Dual-Label Time-Resolved Fluorometric Immunoassay for the Simultaneous Detection of Two Recombinant Proteins in Potato. J Agric Food Chem 48, 5868-5873 (2000).
3. Hardcastle, A. et al. A Duplexed Phenotypic Screen for the Simultaneous Detection of Inhibitors of the Molecular Chaperone Heat Shock Protein 90 and Modulators of Cellular Acetylation. Mol Cancer Ther 6, 1112-1122 (2007).
4. Hemmilä, I., Holttinen, S., Pettersson, K. & Lövgren, T. Double-Label Time-Resolved Immunofluorometry of Lutropin and Follitropin in Serum. Clin Chem 33, 2281-2283 (1987).
5. Xu, Y.Y. et al. Simultaneous Quadruple-Label Fluorometric Immunoassay of Thyroid-Stimulating Hormone, 17 Alpha-Hydroxyprogesterone, Immunoreactive Trypsin, and Creatine Kinase MM Isoenzyme in Dried Blood Spots. Clin Chem 38, 2038-2043 (1992).
6. Zerwes, H. et al. A Multiparameter Screening Assay to Assess the Cytokine-Induced Expression of Endothelial Cell Adhesion Molecules. Anal Biochem 304, 166-173 (2002).

Revvity technical publications

1. Vikstrom, S., Korte, M., Pulli, P., Hurskainen, P. & Gripenberg-Lerche, C. A Cell-Based, Microplate Format, DELFIA Assay for Determination of the Activation of MAP Kinase. Revvity Poster. 
2. Vikstrom, S., Pulli, P., Hurskainen, P. & Gripenberg-Lerche, C. Multiplexing of DELFIA Cell Proliferation and DNA Fragmentation Assays with Conventional Fluorometric Cell-Based Assays for Identification of Toxic Compounds. Revvity Poster. 

Enzymatic assays

Journal articles

1. García-Martínez, L.F. et al. In Vitro High-Throughput Screening Assay for Modulators of Transcription. Anal Biochem 301, 103-110 (2002).
2. Gottlin, E.B. et al. High-Throughput Screen for Inhibitors of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase by Surrogate Ligand Competition. J Biomol Screen 8, 332-339 (2003).
3. Räbinä, J., Pikkarainen, M., Miyasaka, M. & Renkonen, R. A Time-Resolved Immunofluorometric Assay of Sialyl Lewis x-Degrading Alpha 2,3-Sialidase Activity. Anal Biochem 258, 362-368 (1998).
4. Räbinä, J., Mattila, P. & Renkonen, R. Two Time-Resolved Fluorometric High-Throughput Assays for Quantitation of GDP-L-Fucose. Anal Biochem 286, 173-178 (2000).
5. Skovbjerg, H. et al. Gliadin is a Good Substrate of Several Transglutaminases: Possible Implication in the Pathogenesis of Coeliac Disease. Scand J Gastroenterol 37, 812-817 (2002).
6. Zhang, L., Schwartz, G., O'Donnell, M. & Harrison, R.K. Development of a Novel Helicase Assay Using Electrochemiluminescence. Anal Biochem 293, 31-37 (2001).

Apoptosis

Journal articles

1. Walzlein, J. et al. The antitumorigenic response of neural precursors depends on subventricular proliferation and age. Stem Cells 26, 2945-2954 (2008).

Revvity technical publications

1. Sippola, K. et al. Cell Based Assay for Studying the Effects of Cytotoxic Agents. Revvity Poster. 
2. Vikstrom, S., Frang, H., Karvinen, J., Hurskainen, P. & Hemmila, I. A Cell-Based DNA Fragmentation Assay in Microplate Format Using DELFIA Technology. Revvity Poster.
3. Vikstrom, S., Pulli, P., Hurskainen, P. & Gripenberg-Lerche, C. Multiplexing of DELFIA Cell Proliferation and DNA Fragmentation Assays with Conventional Fluorometric Cell-Based Assays for Identification of Toxic Compounds. Revvity Poster. 

Post-translational modification

Journal articles

1. Yuan, Y. et al. A Small-Molecule Probe of the Histone Methyltransferase G9a Induces Cellular Senescence in Pancreatic Adenocarcinoma. ACS chemical biology (2012).
2. Xie, W., Ames, R. S. & Li, H. A Cell-Based High-Throughput Screening Assay to Measure Cellular Histone H3 Lys27 Trimethylation with a Modified Dissociation-Enhanced Lanthanide Fluorescent Immunoassay. Journal of Biomolecular Screening (2011).
3. Boisclair, M.D. et al. Development of a Ubiquitin Transfer Assay for High Throughput Screening by Fluorescence Resonance Energy Transfer. J Biomol Screen 5, 319-328 (2000).
4. Heinke, R. et al. Virtual Screening and Biological Characterization of Novel Histone Arginine Methyltransferase PRMT1 Inhibitors. ChemMedChem 4, 69-77 (2009).
5. Kubicek, S. et al. Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase. Mol. Cell 25, 473-481 (2007).
6. Wynne Aherne, G., Rowlands, M.G., Stimson, L. & Workman, P. Assays for the Identification and Evaluation of Histone Acetyltransferase Inhibitors. Methods 26, 245-253 (2002).

Receptor-ligand binding

Journal articles

Eu-ligand binding (cell membrane-based)

1. Robertson, C. R., Pruess, T. H., Grussendorf, E., White, H. S. & Bulaj, G. Generating Orally Active Galanin Analogues with Analgesic Activities. ChemMedChem (2012).
2. Gao, X. et al. Europium-Labeled Melanin-Concentrating Hormone Analogues: Ligands for Measuring Binding to Melanin-Concentrating Hormone Receptors 1 and 2. Anal Biochem 328, 187-195 (2004).
3. Lee, S. et al. Development of a Time-Resolved Fluorometric Assay for the High Throughput Screening of Melanin Concentrating Hormone Receptor Antagonists. J Pharmacol Toxicol Methods 53, 242-247 (2006).
4. Oh, K. et al. Melanin-Concentrating Hormone-1 Receptor Antagonism and Anti-Obesity Effects of Ethanolic Extract from Morus alba Leaves in Diet-Induced Obese Mice. J Ethnopharmacol 122, 216-220 (2009).
5. Valenzano, K.J. et al. Development of a Fluorescent Ligand-Binding Assay Using the AcroWell Filter Plate. J Biomol Screen 5, 455-461 (2000).

Eu-ligand binding (whole cell-based)

1. Shabanpoor, F. et al. Minimization of relaxin-3 leading to high affinity analogues with increased selectivity for relaxin-family peptide 3 receptor (RXFP3) over RXFP1. Journal of Medicinal Chemistry (2012).
2. Greenwalt, D.E., Szabo, J. & Manchel, I. High Throughput Cell-Based Assay of Hemaoietic Progenitor Differentiation. J Biomol Screen 6, 383-392 (2001).
3. Handl, H.L., Vagner, J., Yamamura, H.I., Hruby, V.J. & Gillies, R.J. Lanthanide-Based Time-Resolved Fluorescence of in Cyto Ligand-Receptor Interactions. Anal Biochem 330, 242-250 (2004).
4. Inglese, J. et al. Chemokine Receptor-Ligand Interactions Measured Using Time-Resolved Fluorescence. Biochemistry 37, 2372-2377 (1998).
5. Li, P., Nagarajan, S., Zhu, C. & Selvaraj, P. Recombinant CD16A-Ig Forms a Homodimer and Cross-Blocks the Ligand Binding Functions of Neutrophil and Monocyte Fc(gamma) Receptors. Mol Immunol 38, 527-538 (2002).

 Revvity technical publications

1. Sippola, K. et al. Europium-labeled Ligands for Receptor Binding Studies. Revvity Poster.
2. Syystö, R. et al. Time-Resolved Fluorometric Receptor Ligand Assay for HTS. Revvity Poster.

Europium labeling

Journal articles

1. Aggerbeck, H., Nørgaard-Pedersen, B. & Heron, I. Simultaneous Quantitation of Diphtheria and Tetanus Antibodies by Double Antigen, Time-Resolved Fluorescence Immunoassay. J Immunol Methods 190, 171-183 (1996).
2. Gao, X. et al. Europium-Labeled Melanin-Concentrating Hormone Analogues: Ligands for Measuring Binding to Melanin-Concentrating Hormone Receptors 1 and 2. Anal Biochem 328, 187-195 (2004).
3. Hurskainen, P. et al. Preparation of Europium-Labelled DNA Probes and Their Properties. Nucl Acids Res 19, 1057-1061 (1991).
4. Mikola, H., Sundell, A.C. & Hänninen, E. Labeling of Estradiol and Testosterone Alkyloxime Derivatives with a Europium Chelate for Time-Resolved Fluoroimmunoassays. Steroids 58, 330-334 (1993).
5. Smith, D.R. et al. Comparison of Dissociation-Enhanced Lanthanide Fluorescent Immunoassays to Enzyme-Linked Immunosorbent Assays for Detection of Staphylococcal Enterotoxin B, Yersinia pestis-Specific F1 Antigen, and Venezuelan Equine Encephalitis Virus. Clin Diagn lab Immun 8, 1070-1075 (2001).
6. Stenroos, K., Hurskainen, P., Blomberg, K. & Lindqvist, C. Solid Phase IL-2-IL-2R Alpha Assay with Time Resolved Fluorometry. Immunol Lett 58, 15-18 (1997).
7. Xu, Y.Y. et al. Simultaneous Quadruple-Label Fluorometric Immunoassay of Thyroid-Stimulating Hormone, 17 Alpha-Hydroxyprogesterone, Immunoreactive Trypsin, and Creatine Kinase MM Isoenzyme in Dried Blood Spots. Clin Chem 38, 2038-2043 (1992).
8. Yamamoto, N. et al. Development of a High-Throughput Fluoroimmunoassay for Syk Kinase and Syk Kinase Inhibitors. Anal Biochem 315, 256-261 (2003).
9. Zerwes, H. et al. A Multiparameter Screening Assay to Assess the Cytokine-Induced Expression of Endothelial Cell Adhesion Molecules. Anal Biochem 304, 166-173 (2002).